한희탁 (연세대학교)

한빛사 논문

조회2,389

연세대학교

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ACS Nano, 2018, 12 (2), pp 932–941. Publication Date (Web): December 20, 2017, DOI: 10.1021/acsnano.7b05826 Single-Droplet Multiplex Bioassay on a Robust and Stretchable Extreme Wetting Substrate through Vacuum-Based Droplet Manipulation

Heetak Han^†, Jung Seung Lee^‡, Hyunchul Kim^†, Sera Shin^†, Jaehong Lee^†, Jongchan Kim^§, Xu Hou^⊥, Seung-Woo Cho^‡ , Jungmok Seo^*∥#, and Taeyoon Lee^*†
^†School of Electrical and Electronic Engineering and ^‡Department of Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea
^§Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, United States
^⊥College of Chemistry and Chemical Engineering, Collaborative Innovation Center of Chemistry for Energy Materials, and State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, China
^∥Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea
^#Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology (UST), Seoul 02792, Republic of Korea

^*Corresponding Authors

Abstract

Herein, a droplet manipulation system with a superamphiphobic (SPO)–superamphiphilic (SPI) patterned polydimethylsiloxane (PDMS) substrate is developed for a multiplex bioassay from single-droplet samples. The SPO substrate is fabricated by sequential spraying of adhesive and fluorinated silica nanoparticles onto a PDMS substrate. It is subsequently subjected to oxygen plasma with a patterned mask to form SPI patterns. The SPO layer exhibits extreme liquid repellency with a high contact angle (>150°) toward low surface tension and viscous biofluidic droplets (e.g., ethylene glycol, blood, dimethyl sulfoxide, and alginate hydrogel). In contrast, the SPI exhibits liquid adhesion with a near zero contact angle. Using the droplet manipulation system, various liquid droplets can be precisely manipulated and dispensed onto the predefined SPI patterns on the SPO PDMS substrate. This system enables a multiplex colorimetric bioassay, capable of detecting multiple analytes, including glucose, uric acid, and lactate, from a single sample droplet. In addition, the detection of glucose concentrations in a plasma droplet of diabetic and healthy mice are performed to demonstrate the feasibility of the proposed system for efficient clinical diagnostic applications.

Keywords: bioassay; biomimetics; colorimetric analysis; droplet manipulation; superamphiphobic

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