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Rice, First Online: 05 January 2018, 11:1 | DOI 10.1186/s12284-017-0196-8
The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves
Abstract
Suk-Hwan Kim1†, Choon-Tak Kwon1,4†, Giha Song1, Hee-Jong Koh1, Gynheung An2 and Nam-Chon Paek1,3*
1Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. 2Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea. 3Crop Biotechnology Institute, Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea. 4Present address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
* Correspondence: Nam-Chon Paek
1Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. 2Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea. 3Crop Biotechnology Institute, Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea. 4Present address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
* Correspondence: Nam-Chon Paek
†Suk-Hwan Kim and Choon-Tak Kwon contributed equally to this work.
Abstract
Background
Rice zebra mutants are leaf variegation mutants that exhibit transverse sectors of green/yellow or green/white in developing or mature leaves. In most cases, leaf variegation is caused by defects in chloroplast biogenesis pathways, leading to an accumulation of reactive oxygen species in a transverse pattern in the leaves. Here, we examine a new type of leaf variegation mutant in rice, zebra3 (z3), which exhibits transverse dark-green/green sectors in mature leaves and lacks the typical yellow or white sectors.
Results
Map-based cloning revealed that the Z3 locus encodes a putative citrate transporter that belongs to the citrate-metal hydrogen symport (CitMHS) family. CitMHS family members have been extensively studied in bacteria and function as secondary transporters that can transport metal-citrate complexes, but whether CitMHS family transporters exist in eukaryotes remains unknown. To investigate whether Z3 acts as a citrate transporter in rice, we measured citrate levels in wild-type leaves and in the dark-green and green sectors of the leaves of z3 mutants. The results showed that citrates accumulated to high levels in the dark-green sectors of z3 mutant leaves, but not in the green sectors as compared with the wild-type leaves.
Conclusions
These results suggest that leaf variegation in the z3 mutant is caused by an unbalanced accumulation of citrate in a transverse pattern in the leaves. Taking these results together, we propose that Z3 plays an important role in citrate transport and distribution during leaf development and is a possible candidate for a CitMHS family member in plants.
Keywords : Rice, ZEBRA3 (Z3), Leaf variegation, CitMHS family, Citrate transporte
논문정보
- Research article
- 2018년 01월 (BRIC 등록일 2018-02-02)
- 국내 연구진
- 생명과학 > 식물학
- 구・농수식품
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