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ACS Nano, Publication Date (Web): December 18, 2017 | DOI: 10.1021/acsnano.7b04321
Electrical Neural Stimulation and Simultaneous in Vivo Monitoring with Transparent Graphene Electrode Arrays Implanted in GCaMP6f Mice
Dong-Wook Park†‡¶ , Jared P. Ness§¶, Sarah K. Brodnick§, Corinne Esquibel§, Joseph Novello§, Farid Atry#, Dong-Hyun Baek§, Hyungsoo Kim†, Jihye Bong†, Kyle I. Swanson⊥∥, Aaron J. Suminski§⊥, Kevin J. Otto□△○, Ramin Pashaie#, Justin C. Williams*§⊥, and Zhenqiang Ma*†
†Department of Electrical and Computer Engineering, §Department of Biomedical Engineering, and ⊥Department of Neurological Surgery, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States
†Department of Electrical and Computer Engineering, §Department of Biomedical Engineering, and ⊥Department of Neurological Surgery, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States
‡ School of Electrical and Computer Engineering, University of Seoul, Seoul 130-743, South Korea
# Department of Electrical Engineering and Computer Science, University of Wisconsin?Milwaukee, Milwaukee, Wisconsin 53706, United States
∥ Barrow Neurological Institute, Phoenix, Arizona 85013, United States
□J. Crayton Pruitt Family Department of Biomedical Engineering, △Department of Neuroscience, and ○Department of Neurology, University of Florida, Gainesville, Florida 32610, United States
*Corresponding Authors
¶D.-W. Park and J. P. Ness contributed equally to this work.
*Corresponding Authors
¶D.-W. Park and J. P. Ness contributed equally to this work.
Abstract
Electrical stimulation using implantable electrodes is widely used to treat various neuronal disorders such as Parkinson’s disease and epilepsy and is a widely used research tool in neuroscience studies. However, to date, devices that help better understand the mechanisms of electrical stimulation in neural tissues have been limited to opaque neural electrodes. Imaging spatiotemporal neural responses to electrical stimulation with minimal artifact could allow for various studies that are impossible with existing opaque electrodes. Here, we demonstrate electrical brain stimulation and simultaneous optical monitoring of the underlying neural tissues using carbon-based, fully transparent graphene electrodes implanted in GCaMP6f mice. Fluorescence imaging of neural activity for varying electrical stimulation parameters was conducted with minimal image artifact through transparent graphene electrodes. In addition, full-field imaging of electrical stimulation verified more efficient neural activation with cathode leading stimulation compared to anode leading stimulation. We have characterized the charge density limitation of capacitive four-layer graphene electrodes as 116.07-174.10 μC/cm2 based on electrochemical impedance spectroscopy, cyclic voltammetry, failure bench testing, and in vivo testing. This study demonstrates the transparent ability of graphene neural electrodes and provides a method to further increase understanding and potentially improve therapeutic electrical stimulation in the central and peripheral nervous systems.
Keywords: electrical stimulation; GCaMP6; graphene electrode; neural electrode; neural implants
Keywords: electrical stimulation; GCaMP6; graphene electrode; neural electrode; neural implants
논문정보
- Research article
- 2017년 12월 (BRIC 등록일 2018-01-17)
- 국내+국외 연구진
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