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Nat. Commun., Published online: 13 December 2017, 8, Article number: 2113 | doi:10.1038/s41467-017-02216-6
Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution
Jae Young Kim1, Eun Seok Seo1, Hyunmin Kim 2, Ji-Won Park3, Dong-Kwon Lim4 & Dae Won Moon1,*
1Department of New Biology, DGIST, Daegu, 42988, Republic of Korea. 2 Companion Diagnostics and Medical Technology Research Group, DGIST, Daegu, 42988, Republic of Korea. 3Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon, 34134, Republic of Korea. 4 KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea.
*Correspondence to Dae Won Moon
1Department of New Biology, DGIST, Daegu, 42988, Republic of Korea. 2 Companion Diagnostics and Medical Technology Research Group, DGIST, Daegu, 42988, Republic of Korea. 3Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, Daejeon, 34134, Republic of Korea. 4 KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea.
*Correspondence to Dae Won Moon
Abstract
We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 μm was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl β-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process.
논문정보
- Research article
- 2017년 12월 (BRIC 등록일 2017-12-20)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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