최은나 (경희대학교) | 한빛사논문 > 한빛사

한빛사논문

조회2,374

경희대학교

CV File 321 kb

Proc. Natl. Acad. Sci. USA, Published online before print September 5, 2017 | doi: 10.1073/pnas.1705437114 A trans-acting leader RNA from a Salmonella virulence gene

Eunna Choi^a,1, Yoontak Han^a,1, Yong-Joon Cho^b,1, Daesil Nam^c, and Eun-Jin Lee^a,2^aDepartment of Genetic Engineering, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, South Korea;

^bUnit of Antarctic K-Route Expedition, Korea Polar Research Institute, Incheon 21990, South Korea;

^cDivision of Microbiology, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Suwon 16419, South Korea
¹E.C., Y.H., and Y.-J.C. contributed equally to this work.

²To whom correspondence should be addressed.

Abstract

Bacteria use flagella to move toward nutrients, find its host, or retract from toxic substances. Because bacterial flagellum is one of the ligands that activate the host innate immune system, its synthesis should be tightly regulated during host infection, which is largely unknown. Here, we report that a bacterial leader mRNA from the mgtCBR virulence operon in the intracellular pathogen Salmonella enterica serovar Typhimurium binds to the fljB coding region of mRNAs in the fljBA operon encoding the FljB phase 2 flagellin, a main component of bacterial flagella and the FljA repressor for the FliC phase 1 flagellin, and degrades fljBA mRNAs in an RNase E-dependent fashion during infection. A nucleotide substitution of the fljB flagellin gene that prevents the mgtC leader RNA-mediated down-regulation increases the fljB-encoded flagellin synthesis, leading to a hypermotile phenotype inside macrophages. Moreover, the fljB nucleotide substitution renders Salmonella hypervirulent, indicating that FljB-based motility must be compromised in the phagosomal compartment where Salmonella resides. This suggests that this pathogen promotes pathogenicity by producing a virulence protein and limits locomotion by a trans-acting leader RNA from the same virulence gene during infection.

flagellin, mgtC, trans-acting leader RNA

논문정보

형식Research article
게재일2017년 09월 (BRIC 등록일 2017-09-11)
연구진국내 연구진
분야 생명과학 > 미생물학

댓글 작성 로그인

CV 열람하기

연락처 보기