한빛사논문
- 조회989
University of California, Berkeley, Lawrence Berkeley National Laboratory, Tsinghua-Berkeley Shenzhen Institute
Ju Hun Lee1-3,7, Christopher M Warner4,7, Hyo-Eon Jin1,2,5,7, Eftihia Barnes6, Aimee R Poda4, Edward J Perkins4 & Seung-Wuk Lee1-3,*
1Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA. 2Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. 3Tsinghua-Berkeley Shenzhen Institute, Berkeley, CA, USA. 4Environmental Laboratory, U.S. Army Engineer Research and Development Center, Vicksburg, MS, USA. 5College of Pharmacy, Ajou University, Suwon, Republic of Korea. 6Geotechnical and Structures Laboratory, U.S. Army Engineer Research and Development Center, Vicksburg, MS, USA. 7These authors contributed equally to this work.
*Correspondence should be addressed to S.-W.L.
Abstract
Large-scale fabrication of precisely defined nanostructures with tunable functions is critical to the exploitation of nanoscience and nanotechnology for production of electronic devices, energy generators, biosensors, and bionanomedicines. Although self-assembly processes have been developed to exploit biological molecules for functional materials, the resulting nanostructures and functions are still very limited, and scalable synthesis is far from being realized. Recently, we have established a bacteriophage-based biomimetic process, called 'self-templating assembly'. We used bacteriophage as a nanofiber model system to exploit its liquid crystalline structure for the creation of diverse hierarchically organized structures. We have also demonstrated that genetic modification of functional peptides of bacteriophage results in structures that can be used as soft and hard tissue-regenerating materials, biosensors, and energy-generating materials. Here, we describe a comprehensive protocol to perform genetic engineering of phage, liter-scale amplification, purification, and self-templating assembly, and suggest approaches for characterizing hierarchical phage nanostructures using optical microscopy, atomic-force microscopy (AFM), and scanning electron microscopy (SEM). We also discuss sources of contamination, common mistakes during the fabrication process, and quality-control measures to ensure reproducible material production. The protocol takes ~8-10 d to complete.
논문정보
- Research article
- 2017년 08월 (BRIC 등록일 2017-09-05)
- 국내+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
