구.농수식품
- 조회1,529
충남대학교
Abstract
Seung Beom Seo a, 1, S.H.S. Dananjaya a, 1, Chamilani Nikapitiya b, c, Bae Keun Park a, Ravi Gooneratne d, Tae-Yoon Kim e, Jehee Lee b, c, Cheol-Hee Kim e, *, Mahanama De Zoysa a, c, **
a College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon, 34134, Republic of
Korea
b Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea c Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea
d Department of Wine, Food and Molecular Biosciences, Faculty of Agriculture & Life Sciences, Lincoln University, New Zealand e Department of Biology, Chungnam National University, Yuseong-gu, Daejeon, 34134, Republic of Korea
* Corresponding author. Department of Biology, Chungnam National University, Yuseong-gu, Daejeon, 34134, Republic of Korea.
** Corresponding author. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea
1These authors contributed equally to this study.
Abstract
Silver nanoparticles (AgNPs) were successfully synthesized by a chemical reduction method, physico-chemically characterized and their effect on wound-healing activity in zebrafish was investigated. The prepared AgNPs were circular-shaped, water soluble with average diameter and zeta potential of 72.66 nm and ?0.45 mv, respectively. Following the creation of a laser skin wound on zebrafish, the effect of AgNPs on wound-healing activity was tested by two methods, direct skin application (2 μg/wound) and immersion in a solution of AgNPs and water (50 μg/L). The zebrafish were followed for 20 days post-wounding (dpw) by visual observation of wound size, calculating wound healing percentage (WHP), and histological examination. Visually, both direct skin application and immersion AgNPs treatments displayed clear and faster wound closure at 5, 10 and 20 dpw compared to the controls, which was confirmed by 5 dpw histology data. At 5 dpw, WHP was highest in the AgNPs immersion group (36.6%) > AgNPs direct application group (23.7%) > controls (18.2%), showing that WHP was most effective in fish immersed in AgNPs solution. In general, exposure to AgNPs induced gene expression of selected wound-healing-related genes, namely, transforming growth factor (TGF-β), matrix metalloproteinase (MMP) ?9 and ?13, pro-inflammatory cytokines (IL-1β and TNF-α) and antioxidant enzymes (superoxide dismutase and catalase), which observed differentiation at 12 and 24 h against the control; but the results were not consistently significant, and many either reached basal levels or were down regulated at 5 dpw in the wounded muscle. These results suggest that AgNPs are effective in acceleration of wound healing and altered the expression of some wound-healing-related genes. However, the detailed mechanism of enhanced wound healing remains to be investigated in fish.
Keywords : Silver nanoparticles, Inflammation, Gene expression, Zebrafish, Wound healing
논문정보
- Research article
- 2017년 09월 (BRIC 등록일 2017-08-21)
- 국내(교신)+국외 연구진
- 의약학 > 면역의학
- 구・농수식품
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