한빛사논문
Ji Sun Park1, Wooram Park2, Sin-jung Park3, Andrew C. Larson2,4,5,6,7, Dong-Hyun Kim2,4 and Keun-Hong Park1,*
1Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Biocomplex, Sampyeong-Dong, Bundang-gu, Seongnam-si, Republic of Korea
2Department of Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA
3 Department of Biopharmaceutical Sciences, College of Pharmacy, University of Illinois, Chicago, IL, USA
4 Robert H. Lurie Comprehensive Cancer Center, Chicago, IL, USA
5 Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA
6 Department of Electrical Engineering and Computer Science, Evanston, IL, USA
7 International Institute of Nanotechnology (IIN), Northwestern University, Evanston, IL, USA
J.S.P. and W.P. contributed equally to this work.
*Corresponding author
Abstract
This study develops multimodal magnetic nanoclusters (M-MNCs) for gene transfer, directed migration, and tracking of human mesenchymal stem cells (hMSCs). The M-MNCs are designed with 5 nm iron oxide nanoparticles and a fluorescent dye (i.e., Rhodamine B) in the matrix of the Food and Drug Administration approved polymer poly(lactide-co-glycolide) using a nanoemulsion method. The synthesized M-MNCs have a hydrodynamic diameter of ~150 nm, are internalized by stem cells via endocytosis, and deliver genes with high efficiency. The cellular internalization and gene expression efficiency of the clustered nanoparticles are significantly higher than that of single nanoparticles. The M-MNC-labeled hMSCs migrate upon application of a magnetic force and can be visualized by both optical and magnetic resonance (MR) imaging. In animal models, the M-MNC-labeled hMSCs are also successfully tracked using optical and MR imaging. Thus, the M-MNCs not only allow the efficient delivery of genes to stem cells but also the tracking of cells in animal models. Taken together, the results show that this new type of nanocomposite can be of great help in future stem cell research and in the development of cell-based therapeutic agents.
Keywords : stem cell tracking; gene delivery; magnetic nanoparticles; magnetic cell migration; tissue engineering
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