한빛사논문
- 조회1,563
Younggeun Park†§, Byunghoon Ryu†§, Bo-Ram Oh†, Yujing Song†, Xiaogan Liang*† , and Katsuo Kurabayashi*†‡
†Department of Mechanical Engineering and ‡Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109, United States
Author Contributions
§Y.P. and B. R. contributed equally.
*Corresponding Authors
Abstract
Monitoring of the time-varying immune status of a diseased host often requires rapid and sensitive detection of cytokines. Metallic nanoparticle-based localized surface plasmon resonance (LSPR) biosensors hold promise to meet this clinical need by permitting label-free detection of target biomolecules. These biosensors, however, continue to suffer from relatively low sensitivity as compared to conventional immunoassay methods that involve labeling processes. Their response speeds also need to be further improved to enable rapid cytokine quantification for critical care in a timely manner. In this paper, we report an immunobiosensing device integrating a biotunable nanoplasmonic optical filter and a highly sensitive few-layer molybdenum disulfide (MoS2) photoconductive component, which can serve as a generic device platform to meet the need of rapid cytokine detection with high sensitivity. The nanoplasmonic filter consists of anticytokine antibody-conjugated gold nanoparticles on a SiO2 thin layer that is placed 170 μm above a few-layer MoS2 photoconductive flake device. The principle of the biosensor operation is based on tuning the delivery of incident light to the few-layer MoS2 photoconductive flake thorough the nanoplasmonic filter by means of biomolecular surface binding-induced LSPR shifts. The tuning is dependent on cytokine concentration on the nanoplasmonic filter and optoelectronically detected by the few-layer MoS2 device. Using the developed optoelectronic biosensor, we have demonstrated label-free detection of IL-1β, a pro-inflammatory cytokine, with a detection limit as low as 250 fg/mL (14 fM), a large dynamic range of 106, and a short assay time of 10 min. The presented biosensing approach could be further developed and generalized for point-of-care diagnosis, wearable bio/chemical sensing, and environmental monitoring.
Keywords: cytokine; immunosensing; localized surface plasmon resonance; molybdenum disulfide; nanoplasmonic filter
논문정보
- Research article
- 2017년 05월 (BRIC 등록일 2017-05-31)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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