양한슬 (The Rockefeller University)

한빛사논문

조회2,597

The Rockefeller University

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Cell, Volume 169, Issue 3, p483–496.e13, 20 April 2017 | http://doi.org/10.1016/j.cell.2017.03.038 Epithelial-Mesenchymal Micro-niches Govern Stem Cell Lineage Choices

Hanseul Yang¹, Rene C. Adam¹, Yejing Ge¹, Zhong L. Hua¹, Elaine Fuchs^{1, 2, 3}¹ Robin Neustein Laboratory of Mammalian Development and Cell Biology, The Rockefeller University, New York, NY 10065, USA
² Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
³ Lead contact: Elaine Fuchs

Corresponding author : Elaine Fuchs

Abstract

Adult tissue stem cells (SCs) reside in niches, which, through intercellular contacts and signaling, influence SC behavior. Once activated, SCs typically give rise to short-lived transit-amplifying cells (TACs), which then progress to differentiate into their lineages. Here, using single-cell RNA-seq, we unearth unexpected heterogeneity among SCs and TACs of hair follicles. We trace the roots of this heterogeneity to micro-niches along epithelial-mesenchymal interfaces, where progenitors display molecular signatures reflective of spatially distinct local signals and intercellular interactions. Using lineage tracing, temporal single-cell analyses, and chromatin landscaping, we show that SC plasticity becomes restricted in a sequentially and spatially choreographed program, culminating in seven spatially arranged unilineage progenitors within TACs of mature follicles. By compartmentalizing SCs into micro-niches, tissues gain precise control over morphogenesis and regeneration: some progenitors specify lineages immediately, whereas others retain potency, preserving self-renewing features established early while progressively restricting lineages as they experience dynamic changes in microenvironment.

논문정보

형식Research article
게재일2017년 04월 (BRIC 등록일 2017-04-18)
연구진국외 연구진
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