한빛사논문
- 조회1,108
Abstract
Yongju Kim, Huichang Li, Ying He, Xi Chen, Xiaoteng Ma and Myongsoo Lee*
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
*Correspondence to : Myongsoo Lee
Hierarchical assemblies of biomolecular subunits can carry out versatile tasks at the cellular level with remarkable spatial and temporal precision1, 2. As an example, the collective motion and mutual cooperation between complex protein machines mediate essential functions for life, such as replication3, synthesis4, degradation5, repair6 and transport7. Nucleic acid molecules are far less dynamic than proteins and need to bind to specific proteins to form hierarchical structures. The simplest example of these nucleic acid-based structures is provided by a rod-shaped tobacco mosaic virus, which consists of genetic material surrounded by coat proteins8. Inspired by the complexity and hierarchical assembly of viruses, a great deal of effort has been devoted to design similarly constructed artificial viruses9, 10. However, such a wrapping approach makes nucleic acid dynamics insensitive to environmental changes. This limitation generally restricts, for example, the amplification of the conformational dynamics between the right-handed B form to the left-handed Z form of double-stranded deoxyribonucleic acid (DNA)11, 12. Here we report a virus-like hierarchical assembly in which the native DNA and a synthetic coat undergo repeated collective helicity switching triggered by pH change under physiological conditions. We also show that this collective helicity inversion occurs during translocation of the DNA-coat assembly into intracellular compartments. Translating DNA conformational dynamics into a higher level of hierarchical dynamics may provide an approach to create DNA-based nanomachines.
논문정보
- Research article
- 2017년 03월 (BRIC 등록일 2017-03-31)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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