한빛사논문
- 조회1,505
Abstract
Dr. James Murray1,†, Jaehwan Sim2,†, Kyunghoon Oh4, Gihyun Sung4, Ara Lee4, Dr. Annadka Shrinidhi1, Dr. Ayyavu Thirunarayanan1, Dr. Dinesh Shetty1,* and Prof. Dr. Kimoon Kim1,2,3,4,*
1 Center for Self-assembly and Complexity (CSC), Institute of Basic Science (IBS), Pohang, Republic of Korea
2 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea
3 Department of Chemistry, Pohang University of Science and Technology, Pohang, Republic of Korea
4 Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang, Republic of Korea
† These authors contributed equally to this work.
*Corresponding authors
Abstract
Chemical proteomics relies primarily on click-chemistry-based protein labeling and biotin-streptavidin enrichment, but these techniques have inherent limitations. Enrichment of intracellular proteins using a totally synthetic host-guest complex is described, overcoming the problem associat?ed with the classical approach. We achieve this by affinity-based protein labeling with a target-specific probe molecule conjugated to a high-affinity guest (suberanilohydroxamic acid-ammonium-adamantane; SAHA-Ad) and then enriching the labeled species using a cucurbit[7]uril bead. This method shows high specificity for labeled molecules in a MDA-MB-231 breast cancer cell lysate. Moreover, this method shows promise for labeling proteins in live cells.
Keywords : cucurbiturils; host-guest chemistry; protein chemistry; protein enrichment; supramolecular chemistry
논문정보
- Research article
- 2017년 02월 (BRIC 등록일 2017-02-08)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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