상위피인용논문
- 조회807
Abstract
Yoon Young Choia, 1, Bong Geun Chungb, 1, Dae Ho Leea, Ali Khademhosseinic, d, Jong-Hoon Kime, Sang-Hoon Leea,*
a Department of Biomedical Engineering, Korea University, Seoul 136-701, Republic of Korea
b Department of Bionano Engineering, Hanyang University, Ansan 426-791, Republic of Korea
c Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA
d Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
e Division of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 136-705, Republic of Korea
1 These authors equally contributed to this work.
*Corresponding author : Sang-Hoon Lee
Abstract
Embryonic stem (ES) cells hold great potential as a renewable cell source for regenerative medicine and cell-based therapy. Despite the potential of ES cells, conventional stem cell culture methods do not enable the control of the microenvironment. A number of microscale engineering approaches have been recently developed to control the extracellular microenvironment and to direct embryonic stem cell fate. Here, we used engineered concave microwell arrays to regulate the size and shape of embryoid bodies (EBs)-cell aggregate intermediates derived from ES cells. Murine ES cells were aggregated within concave microwells, and their aggregate sizes were controlled by varying the microwell widths (200, 500, and 1000 μm). Differentiation of murine ES cells into three germ layers was assessed by analyzing gene expression. We found that ES cell-derived cardiogenesis and neurogenesis were strongly regulated by the EB size, showing that larger concave microwell arrays induced more neuronal and cardiomyocyte differentiation than did smaller microwell arrays. Therefore, this engineered concave microwell array could be a potentially useful tool for controlling ES cell behavior.
Keywords : Concave microwell array; Embryonic stem cell differentiation; Neurogenesis; Cardiogenesis
논문정보
- Research article
- 2010년 05월 (BRIC 등록일 2016-12-20)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
- 120회 이상 인용된 논문
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