Rohidas Arote (서울대학교) | 한빛사논문 > 한빛사

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Rohidas AroteRohidas Arote

서울대학교

Biomaterials, Volume 28, Issue 4, February 2007, Pages 735-744 | http://dx.doi.org/10.1016/j.biomaterials.2006.09.028 A biodegradable poly(ester amine) based on polycaprolactone and polyethylenimine as a gene carrier

Abstract

Rohidas Arote^a, Tae-Hee Kim^a, You-Kyoung Kim^a, Soon-Kyung Hwang^b, Hu-Lin Jiang^a, Ho-Hyun Song^a, Jae-Woon Nah^c, Myung-Haing Cho^b, Chong-Su Cho^a,*^a School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea
^b College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea
^c Department of Polymer Science and Engineering, Sunchon National University, Sunchon 540-742, South Korea

^*Corresponding author : Chong-Su Cho

Abstract
The aim of research was to develop and optimize delivery systems for plasmid DNA (pDNA) based on biodegradable polymers, in particular, poly(ester amine)s (PEAs), suitable for non-viral gene therapy. Poly(ester amine)s were successfully synthesized by Michael addition reaction between polycaprolactone (PCL) diacrylate and low molecular weight polyethylenimine (PEI). PEA/DNA complexes showed effective and stable DNA condensation with the particle sizes below 200 nm, implicating its potential for intracellular delivery. PEAs showed controlled degradation and were essentially non-toxic in all three cells (293 T: Human kidney carcinoma, HepG2: Human hepatoblastoma and HeLa: Human cervix epithelial carcinoma cell lines) at higher doses in contrast to PEI 25 K. PEAs also revealed much higher transfection efficiencies in three cell lines as compared to PEI 25 K. The highest reporter gene expression was observed for PCL/PEI-1.2 (MW 1200) complex having transfection efficiency 15?25 folds higher than PEI 25 K in vitro. Also PEA/DNA complexes successfully transfected cells in vivo after aerosol administration than PEI 25 K. These PEAs can be used as most efficient polymeric vectors which provide a versatile platform for further investigation of structure property relationship along with the controlled degradation, significant low cytotoxicity and high transfection efficiency.

Keywords : Gene delivery; Degradable polymers; Poly(ester amine)s; Polycaprolactone; Polyethylenimine

논문정보

형식Research article
게재일2007년 02월 (BRIC 등록일 2016-11-28)
연구진국내 연구진
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피인용횟수120회 이상 인용된 논문

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