구.농수식품
- 조회2,801
Abstract
Woonsung Na1†, Kwang-Soo Lyoo2†, Sun-Woo Yoon3†, Minjoo Yeom1, Bokyu Kang4, Hyoungjoon Moon4, Hye Kwon Kim2, Dae Gwin Jeong2, Jeong-Ki Kim1* and Daesub Song1*
1 College of Pharmacy, Korea University, Sejong, Republic of Korea. 2 Korea Zoonosis Research Institute, Chonbuk National University, Iksan, Republic of Korea. 3 Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. 4 Research Unit, Green Cross Veterinary Products, Yong.in, Republic of Korea. †Woonsung Na, Kwang-Soo Lyoo and Sun-Woo Yoon contributed equally to this work
*Correspondence : Jeong.Ki Kim, Daesub Song
Abstract
Equine influenza virus (EIV) causes a highly contagious disease in horses and other equids. Recently, we isolated an H3N8 EIV (A/equine/Kyonggi/SA1/2011) from a domestic horse in South Korea that exhibited symptoms of respiratory disease, and found that the EIV strain contained a naturally mutated NS gene segment encoding a truncated NS1 protein. In order to determine whether there was an association between the NS gene truncation and viral virulence, a reverse genetics system was applied to generate various NS gene recombinant viruses using the backbone of the H1N1 A/Puerto Rico/8/1934 (PR/8) virus. In a mouse model, the recombinant PR/8 virus containing the mutated NS gene of the Korean H3N8 EIV strain showed a dramatically reduced virulence: it induced no weight loss, no clinical signs and no histopathological lesions. However, the mice infected with the recombinant viruses with NS genes of PR/8 and H3N8 A/equine/2/Miami/1963 showed severe clinical signs including significant weight loss and 100% mortality. In addition, the levels of the pro-inflammatory cytokines; IL-6, CCL5, and IFN-γ, in the lungs of mice infected with the recombinant viruses expressing a full-length NS1 were significantly higher than those of mice infected with the virus with the NS gene from the Korean H3N8 EIV strain. In this study, our results suggest that the C-terminal moiety of NS1 contains a number of virulence determinants and might be a suitable target for the development of a vaccine candidate against equine influenza.
논문정보
- Research article
- 2016년 11월 (BRIC 등록일 2016-11-23)
- 국내 연구진
- 생명과학 > 미생물학
- 구・농수식품
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