한빛사논문
- 조회1,162
Abstract
Dae InKim1 and KyleJ.Roux1,2,*
1Sanford Children's Health Research Center, Sanford Research, Sioux Falls, SD 57104, USA
2Department of Pediatrics, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD 57105, USA
*Correspondence : KyleJ.Roux
Summary
There are inherent limitations with traditional methods to study protein behavior or to determine the constituency of proteins in discrete subcellular compartments. In response to these limitations, several methods have recently been developed that use proximity-dependent labeling. By fusing proteins to enzymes that generate reactive molecules, most commonly biotin, proximate proteins are covalently labeled to enable their isolation and identification. In this review we describe current methods for proximity-dependent labeling in living cells and discuss their applications and future use in the study of protein behavior.
Keywords: proximity-dependent labeling, APEX, BioID, protein-protein interactions, subcellular proteome, proteomics
논문정보
- Review Paper
- 2016년 09월 (BRIC 등록일 2016-09-27)
- 국외 연구진
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