한빛사논문
- 조회1,667
Abstract
Myeong Ok Cho1,2,7, Zhengzheng Li1,3,7, Hye-Eun Shim2,7, Ik-Sung Cho1, Md Nurunnabi1, Honghyun Park4, Kuen Yong Lee4, Sung-Hwan Moon5, Ki-Suk Kim2, Sun-Woong Kang2,6,8 and Kang Moo Huh1,8
1Department of Polymer Science and Engineering, Chungnam National University, Daejeon, Republic of Korea
2Next-generation Pharmaceutical Research Center, Korea Institute of Toxicology, Daejeon, Republic of Korea
3School of Chemical Engineering and Materials, Tianjin University of Science & Technology, Tianjin, China
4Department of Bioengineering, Hanyang University, Seoul, Republic of Korea
5Department of Medicine, School of Medicine, Konkuk University, Seoul, Republic of Korea
6Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon, Republic of Korea
7These authors contributed equally to this paper as first authors.
8These authors contributed equally to this paper as corresponding authors.
Correspondence: Dr S-W Kang, Next-generation Pharmaceutical Research Center, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea. Professor KM Huh, Department of Polymer Science and Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea.
Abstract
Three-dimensional (3D) cell culture systems have promising applications compared with conventional two-dimensional cell culture systems. Herein, we report a facile method for the formation of 3D spheroids using novel thermo-reversible polysaccharide-based hydrogels. A series of thermo-reversible hydrogels consisting of N-acyl glycol chitosans (NAGCs) are synthesized through a simple N-acylation reaction, and the degree of acylation is finely tuned to obtain adequate thermo-reversible properties and gel stability. Among the NAGCs, N-hexanoyl glycol chitosan is the most thermo-sensitive and is highly effective for forming multi-cellular spheroids when used to coat the surfaces of cell culture dishes. Cell spheroids are effectively formed at various cell concentrations, and their spheroid shape and cellular functions are well maintained for longer times. The hydrogel culture system is also useful for co-cultures that mimic a biological microenvironment. Our thermo-reversible hydrogels may offer a convenient method for the development of in vitro 3D cell culture systems to provide enhanced performance in tissue regeneration, organ-on-chips, drug screening research and other biomedical applications.
논문정보
- Research article
- 2016년 09월 (BRIC 등록일 2016-09-20)
- 국내(교신)+국외 연구진
- 의약학 > 재생의학
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