구.농수식품
부산대학교
Abstract
Dong Jung Leea, 1, Jun Beom Leea, 1, Ho Am Janga, Dominique Ferrandonb, Bok Luel Leea
a Global Research Laboratory, College of Pharmacy, Pusan National University, Busan 46241, South Korea
b Equipe Fondation Recherche Me´dicale, UPR 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire du CNRS, Strasbourg, France
1 Both authors contributed equally to this work.
Corresponding author : Bok Luel Lee
Abstract
Recently, our group demonstrated that the bean bug, Riptortus pedestris, is a good experimental symbiosis model to study the molecular cross-talk between the host insect and the gut symbiont. The Burkholderia symbiont is orally acquired by host nymphs from the environment in every generation. However, it is still unclear how Riptortus specifically interacts with entomopathogens that are abundant in the environmental soil. In preliminary experiments, we observed that a potent entomopathogen, Serratia marcescens, can colonize the midgut of Riptortus insects and was recovered from the midgut when Serratia cells were orally administered, suggesting that this pathogenic bacterium can escape host immune defenses in the salivary fluid. We examined how orally fed Serratia cells can survive in the presence of antimicrobial substances of the Riptortus salivary fluid. In this study, a 15 kDa trialysin-like protein from the salivary gland of R. pedestris and a potent virulence factor of Serratia cells, a serralysin metalloprotease, from the culture medium of S. marcescens were successfully purified to homogeneity. When the purified Riptortus trialysin (rip-trialysin) was incubated with purified serralysin, rip-trialysin was specifically hydrolyzed by serralysin, leading to the loss of antimicrobial activity. These results clearly demonstrated that a potent virulent metalloprotease of S. marcescens functions as a key player in the escape from the salivary fluid-mediated host immune response, resulting in successful colonization of S. marcescens in the host midgut.
Keywords : Rip-trialysin peptide; Serralysin; Pathogen-host interaction; Serratia marcescens; Riptortus pedestris
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