한빛사논문, 상위피인용논문
- 조회2,951
Abstract
Nambin Yim1, Seung-Wook Ryu2, Kyungsun Choi1, Kwang Ryeol Lee2, Seunghee Lee1, Hojun Choi1, Jeongjin Kim3, Mohammed R. Shaker4, Woong Sun4, Ji-Ho Park1, Daesoo Kim3, Won Do Heo3,5,6 & Chulhee Choi1,2,5
1 Department of Bio and Brain Engineering, KAIST, Daejeon 34141, Korea. 2 Cellex Life Sciences Inc., Daejeon 34141, Korea. 3 Department of Biological Sciences, KAIST, Daejeon 34141, Korea. 4 Department of Anatomy, Brain Korea 21 Program, Korea University College of Medicine, Seoul 02841, Korea. 5 Cancer Metastasis Control Center, KAIST Institute for the Biocentury, KAIST, Daejeon 34141, Korea. 6 Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon 34047, Korea.
Correspondence to : Kyungsun Choi or Chulhee Choi
Abstract
Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named ‘exosomes for protein loading via optically reversible protein-protein interactions’ (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues.
논문정보
- Research article
- 2016년 07월 (BRIC 등록일 2016-07-27)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
- 60회 이상 인용된 논문 (상위피인용논문 등록일 2020-04-20)
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