구.농수식품
- 조회1,403
서울대학교
Abstract
Li Liu1, Jelli Venkatesh1, Yeong Deuk Jo1, Sota Koeda2, Munetaka Hosokawa2, Jin-Ho Kang3, Sandra Goritschnig4, Byoung-Cheorl Kang1,*
1 Department of Plant Science and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea
2 Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan
3 Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, 232-916, Korea
4 Keygene N.V., P.O. Box 216, 6700 AE, Wageningen, The Netherlands
*Corresponding author
Abstract
Seychelles-2 (‘sy-2’) is a temperature-sensitive natural mutant of Capsicum chinense, which exhibits an abnormal leaf phenotype when grown at temperatures below 24 °C. We previously showed that the sy-2 phenotype is controlled by a single recessive gene, sy-2, located on pepper chromosome 1. In this study, a high-resolution genetic and physical map for the sy-2 locus was constructed using two individual F2 mapping populations derived from a cross between C. chinense mutant ‘sy-2’ and wild-type ‘No. 3341’. The sy-2 gene was fine mapped to a 138.8-kb region between markers SNP 5-5 and SNP 3-8 at the distal portion of chromosome 1, based on comparative genomic analysis and genomic information from pepper. The sy-2 target region was predicted to contain 27 genes. Expression analysis of these predicted genes showed a differential expression pattern for ORF10 and ORF20 between mutant and wild-type plants; with both having significantly lower expression in ‘sy-2’ than in wild-type plants. In addition, the coding sequences of both ORF10 and ORF20 contained single nucleotide polymorphisms (SNPs) causing amino acid changes, which may have important functional consequences. ORF10 and ORF20 are predicted to encode F-box proteins, which are components of the SCF complex. Based on the differential expression pattern and the presence of nonsynonymous SNPs, we suggest that these two putative F-box genes are most likely responsible for the temperature-sensitive phenotypes in pepper. Further investigation of these genes may enable a better understanding of the molecular mechanisms of low temperature sensitivity in plants.
논문정보
- Research article
- 2016년 08월 (BRIC 등록일 2016-08-01)
- 국내(교신)+국외 연구진
- 생명과학 > 유전학
- 구・농수식품
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