한빛사논문
경북대학교 의과대학
Abstract
Dr. Swarbhanu Sarkar1,†, Yeong Su Ha1,†, Nisarg Soni1,†, Dr. Gwang Il An2,†, Woonghee Lee1, Min Hwan Kim2, Phuong Tu Huynh1, Heesu Ahn1, Dr. Nikunj Bhatt1, Dr. Yong Jin Lee2, Dr. Jung Young Kim2, Prof. Kwon Moo Park1, Prof. Isao Ishii3, Prof. Shin-Geol Kang4 and Prof. Jeongsoo Yoo1,*
1 Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, Korea
2 Molecular Imaging Research Centre, Korea Institute of Radiological and Medical Sciences, Seoul, Korea
3 Department of Biochemistry, Keio University, Tokyo, Japan
4 Department of Chemistry, Daegu University, Gyeongsan, Korea
† These authors contributed equally.
*Corresponding author
Abstract
Hydrogen sulfide (H2S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2S. Macrocyclic 64Cu complexes that instantly reacted with gaseous H2S to form insoluble 64CuS in a highly sensitive and selective manner were prepared. The H2S concentration in biological samples was measured by a thin-layer radiochromatography method. When 64Cu-cyclen was injected into mice, an elevated H2S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2S levels in a millimeter-sized infarcted lesion of the rat heart.
Keywords : gas immobilization; hydrogen sulfide; imaging agents; nuclear imaging; radioisotopes
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