정호성 (연세대학교)

한빛사논문, 상위피인용논문

조회5,033

연세대학교

CV File 86 kb

Cell, 166, 1-12, June 30, 2016 | doi:10.1016/j.cell.2016.05.029 Dynamic Axonal Translation in Developing and Mature Visual Circuits

Abstract

Toshiaki Shigeoka,^1,4 Hosung Jung,^2,4,5,* Jane Jung,² Benita Turner-Bridger,¹ Jiyeon Ohk,² Julie Qiaojin Lin,¹ Paul S. Amieux,³ and Christine E. Holt^1,5,*

¹Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
²Department of Anatomy, Brain Research Institute, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
³Bastyr University Research Institute, Bastyr University, Kenmore, WA 98028, USA
⁴Co-first author
⁵Co-senior author

^*Correspondence:Hosung Jung, Christine E. Holt

Summary
Local mRNA translation mediates the adaptive responses of axons to extrinsic signals, but direct evidence that it occurs in mammalian CNS axons in vivo is scant. We developed an axon-TRAP-RiboTag approach in mouse that allows deep-sequencing analysis of ribosome-bound mRNAs in the retinal ganglion cell axons of the developing and adult retinotectal projection in vivo. The embryonic-to-postnatal axonal translatome comprises an evolving subset of enriched genes with axon-specific roles, suggesting distinct steps in axon wiring, such as elongation, pruning, and synaptogenesis. Adult axons, remarkably, have a complex translatome with strong links to axon survival, neurotransmission, and neurodegenerative disease. Translationally co-regulated mRNA subsets share common upstream regulators, and sequence elements generated by alternative splicing promote axonal mRNA translation. Our results indicate that intricate regulation of compartment-specific mRNA translation in mammalian CNS axons supports the formation and maintenance of neural circuits in vivo.

논문정보

형식Research article
게재일2016년 06월 (BRIC 등록일 2016-06-22)
연구진국내(교신)+국외 연구진
분야 의약학 > 분자세포의학
피인용횟수60회 이상 인용된 논문 (상위피인용논문 등록일 2019-04-15)

댓글 작성 로그인

CV 열람하기

연락처 보기