상위피인용논문
- 조회816
고려대학교
Abstract
Yeon Seok Kima, Ho Sup Jungb, Toshihiko Matsuurab, Hea Yeon Leeb,*, Tomoji Kawaib, Man Bock Gua,*
a College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea
b Institute for Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
*Corresponding authors : Hea Yeon Lee, Man Bock Gu
Abstract
An electrochemical detection method for chemical sensing has been developed using a DNA aptamer immobilized gold electrode chip. DNA aptamers specifically binding to 17β-estradiol were selected by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random ssDNA library, composed of approximately 7.2 × 1014 DNA molecules. Gold electrode chips were employed to evaluate the electrochemical signals generated from interactions between the aptamers and the target molecules. The DNA aptamer immobilization on the gold electrode was based on the avidin?biotin interaction. The cyclic voltametry (CV) and square wave voltametry (SWV) values were measured to evaluate the chemical binding to aptamer. When 17β-estradiol interacted with the DNA aptamer, the current decreased due to the interference of bound 17β-estradiol with the electron flow produced by a redox reaction between ferrocyanide and ferricyanide. In the negative control experiments, the current decreased only mildly due to the presence of other chemicals.
Keywords : Electrochemical detection; Aptamer; 17β-Estradiol
논문정보
- Research article
- 2007년 05월 (BRIC 등록일 2016-06-10)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
- 120회 이상 인용된 논문
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