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Fish Shellfish Immunol., Available online 15 May 2016 | doi:10.1016/j.fsi.2016.05.018 A prebiotic role of Ecklonia cava improves the mortality of Edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria

Abstract

WonWoo Lee^a, Jae Young Oh^a, Eun-A. Kim^a, Nalae Kang^a, Kil-Nam Kim^b, Ginnae Ahn^c,1,* You-Jin Jeon^{a, 1,*}

^a Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea
^b Marine Bio Research Team, Korea Basic Science Institute (KBSI), 690-140, Republic of Korea
^c Department of Marine Bio-food Science, College of Fisheries and Ocean Sciences, Chonnam National University, 500-749, Republic of Korea
¹ G.A and Y.J.J equally contributed to this study.
^*Corresponding author : Ginnae Ahn, You-Jin Jeon

Abstract
In this study, the beneficial prebiotic roles of Ecklonia cava (E. cava, EC) were evaluated on the growth of lactic acid bacteria (LAB) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. The result showed that the original E. cava (EC) led to the highest growth effects on three LABs (Lactobacillus brevis, L. brevis; Lactobacillus pentosus, L. pentosus; Lactobacillus plantarum; L. plantarum) and it was dose-dependent manners. Also, EC, its Celluclast enzymatic (ECC) and 100% ethanol extracts (ECE) showed the anti-bacterial activities on the fish pathogenic bacteria such as (Edwardsiella tarda; E. tarda, Streptococcus iniae; S. iniae, and Vibrio harveyi; V. harveyi). Interestingly, EC induced the higher production of the secondary metabolites from L. plantarum in MRS medium. The secondary metabolites produced by EC significantly inhibited the growth of pathogen bacteria. In further in vivo study, the co-treatment of EC and L. plantarum improved the growth and mortality of E. tarda-infected zebrafish as regulating the expression of inflammatory molecules such as iNOS and COX2. Taken together, our present study suggests that the EC plays an important role as a potential prebiotic and has a protective effect against the infection caused by E. tarda injection in zebrafish. Also, our conclusion from this evidence is that EC can be used and applied as a useful prebiotic.

Keywords : Ecklonia cava; Prebiotics; Secondary metabolites; Edwardsiella tarda-Infetcted zebrafish; Inflammation mediator

논문정보

형식Research article
게재일2016년 05월 (BRIC 등록일 2016-05-19)
연구진국내 연구진
분야 생명과학 > 면역학
구분구・농수식품

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