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Transbound. Emerg. Dis., Article first published online: 10 MAR 2016 | DOI: 10.1111/tbed.12490 Detection and Phylogenetic Analysis of Porcine Deltacoronavirus in Korean Swine Farms, 2015

Abstract

J. H. Lee^1,†, H. C. Chung^1,†, V. G. Nguyen^2,†, H. J. Moon³, H. K. Kim⁴, S. J. Park⁵, C. H. Lee¹, G. E. Lee¹ and B. K. Park^1,*

¹Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea

²Department of Veterinary Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam

³ Research Unit, Green Cross Veterinary Products, Yongin, Korea

⁴Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea

⁵Forensic Medicine Division, Daegu Institute, National Forensic Service, Chilgok, Korea

^†These authors have contributed equally to this study.

^* Correspondence: B. K. Park. Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University DaeHakRo 1, GwanAk-Gu, Seoul 151-742, Korea.

Summary

This study applied molecular-based method to investigate the presence of porcine deltacoronavirus (PDCoV) in 59 commercial pig farms in South Korea. The results of RT-PCR screening on a relatively large collection of faeces samples (n = 681) from January 2013 to March 2015 did not reveal the presence of PDCoV until the end of 2014. However, on March 2015, PDCoV-positive samples (SL2, SL5) were detected from SL swine farm in Gyeongbuk province. The phylogenetic trees based on the complete spike- and nucleocapsid protein-coding genes showed that SL2 and SL5 closely related to the US PDCoV strains rather than those in China. Thought Korean strains of PDCoV isolated in 2014 (KNU14.04) and in 2015 (SL2 and SL5) grouped within US PDCoV cluster, the reconstruction of ancestral amino acid changes suggested that they are different.

Keywords : porcine deltacoronavirus; swine; South Korea

논문정보

형식Research article
게재일2016년 03월 (BRIC 등록일 2016-03-18)
연구진국내(교신)+국외 연구진
분야 의약학 > 면역의학
구분구・농수식품

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