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KAIST, 현 연세대학교

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J. Biol. Chem, 278, 42377-42385. | doi: 10.1074/jbc.M307561200 Interaction of the ERC Family of RIM-binding Proteins with the Liprin-α Family of Multidomain Proteins

Abstract

Jaewon Ko, Moonseok Na, Seho Kim, Jae-Ran Lee and Eunjoon Kim^‡

National Creative Research Initiative Center for Synaptogenesis and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea

^‡ To whom correspondence should be addressed: Dept. of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.

Abstract

Liprin-α/SYD-2 is a family of multidomain proteins with four known isoforms. One of the reported functions of liprin-α is to regulate the development of presynaptic active zones, but the underlying mechanism is poorly understood. Here we report that liprin-α directly interacts with the ERC (ELKS-Rab6-interacting protein-CAST) family of proteins, members of which are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. In vitro results indicate that ERC2/CAST, an active zone-specific isoform, interacts with all of the known isoforms of liprin-α and that liprin-α1 associates with both ERC2 and ERC1b, a splice variant of ERC1 that distributes to both cytosolic and active zone regions. ERC2 colocalizes with liprin-α1 in cultured neurons and forms a complex with liprin-α1 in brain. Liprin-α1, when expressed alone in cultured neurons, shows a partial synaptic localization. When coexpressed with ERC2, however, liprin-α1 is redistributed to synaptic sites. Moreover, roughly the first half of ERC2, which contains the liprin-α-binding region, is sufficient for the synaptic localization of liprin-α1 while the second half is not. These results suggest that the interaction between ERC2 and liprin-α may be involved in the presynaptic localization of liprin-α and the molecular organization of presynaptic active zones.

논문정보

형식Research article
게재일2003년 10월 (BRIC 등록일 2016-02-15)
연구진국내 연구진
분야 생명과학 > 생리학
피인용횟수120회 이상 인용된 논문

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