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University of California, Berkeley, Lawrence Berkeley National Laboratory

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Nano Lett., Publication Date (Web): October 12, 2015 | DOI: 10.1021/acs.nanolett.5b03678 Selective and Sensitive Sensing of Flame Retardant Chemicals Through Phage Display Discovered Recognition Peptide

Hyo-Eon Jin^†§, Chris Zueger^‡§, Woo-Jae Chung^†§, Winnie Wong^†§, Byung Yang Lee^*†§∥, and Seung-Wuk Lee^*†§

^†Department of Bioengineering and ‡Department of Mechanical Engineering, University of California, Berkeley, California 94720, United States

^§Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States

^∥Department of Mechanical Engineering, Korea University, Seoul 136-713, Korea

^*Corresponding authors

Abstract

We report a highly selective and sensitive biosensor for the detection of an environmentally toxic molecule, decabrominated diphenyl ether (DBDE), one of the most common congeners of the polybrominated frame retardants (polybrominated diphenyl ether (PBDE)), using newly discovered DBDE peptide receptors integrated with carbon nanotube field-effect transistors (CNT-FET). The specific DBDE peptide receptor was identified using a high-throughput screening process of phage library display. The resulting binding peptide carries an interesting consensus binding pocket with two Trp-His/Asn-Trp repeats, which binds to the DBDE in a multivalent manner. We integrated the novel DBDE binding peptide onto the CNT-FET using polydiacetylene coating materials linked through cysteine-maleimide click chemistry. The resulting biosensor could detect the desired DBDE selectively with a 1 fM detection limit. Our combined approaches of selective receptor discovery, material nanocoating through click chemistry, and integration onto a sensitive CNT-FET electronic sensor for desired target chemicals will pave the way toward the rapid development of portable and easy-to-use biosensors for desired chemicals to protect our health and environment.

Keywords: Phage display; polybrominated diphenyl ether; environmental toxicant; biosensor

논문정보

형식Research article
게재일2015년 10월 (BRIC 등록일 2015-10-19)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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