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한빛사논문

조회1,707

University of California Berkeley

Nat. Mater., Published online 27 July 2015 | doi:10.1038/nmat4342 Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces

Abstract

Hojeong Jeon^1,2†, Sangmo Koo^1†, Willie Mae Reese³, Peter Loskill^3,4, Costas P. Grigoropoulos^1* and Kevin E. Healy^3,4*

¹Laser Thermal Laboratory, Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA. ²Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea. ³Department of Materials Science and Engineering, University of California, Berkeley, California 94720, USA. ⁴Department of Bioengineering and California Institute for Quantitative Biosciences (QB3), University of California at Berkeley, Berkeley, California 94720, USA. ^†These authors contributed equally to this work.

Correspondence to : Costas P. Grigoropoulos or Kevin E. Healy

Although adhesive interactions between cells and nanostructured interfaces have been studied extensively^{1, 2, 3, 4, 5, 6}, there is a paucity of data on how nanostructured interfaces repel cells by directing cell migration and cell-colony organization. Here, by using multiphoton ablation lithography⁷ to pattern surfaces with nanoscale craters of various aspect ratios and pitches, we show that the surfaces altered the cells’ focal-adhesion size and distribution, thus affecting cell morphology, migration and ultimately localization. We also show that nanocrater pitch can disrupt the formation of mature focal adhesions to favour the migration of cells towards higher-pitched regions, which present increased planar area for the formation of stable focal adhesions. Moreover, by designing surfaces with variable pitch but constant nanocrater dimensions, we were able to create circular and striped cellular patterns. Our surface-patterning approach, which does not involve chemical treatments and can be applied to various materials, represents a simple method to control cell behaviour on surfaces.

논문정보

형식Research article
게재일2015년 07월 (BRIC 등록일 2015-08-04)
연구진국내+국외 연구진
분야 바이오·의료융합 > 바이오소재

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