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Nat. Commun., Published 26 May 2015, 6, Article number: 7238 | doi:10.1038/ncomms8238
Real-time intermembrane force measurements and imaging of lipid domain morphology during hemifusion
Abstract
Dong Woog Lee1,*, Kai Kristiansen1,*, Stephen H. Donaldson, Jr.1, Nicholas Cadirov1, Xavier Banquy2 & Jacob N. Israelachvili1,3
1 Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA. 2Canada Research Chair in Bio-inspired Materials and Interfaces, Faculty of Pharmacy, Université de Montréal, C.P. 6128, Succursale Centre Ville, Montréal, Quebec H3C 3J7, Canada. 3 Department of Materials, University of California, Santa Barbara, California 93106, USA. * These authors contributed equally to this work.
Correspondence to : Dong Woog Lee or Jacob N. Israelachvili
Abstract
Membrane fusion is the core process in membrane trafficking and is essential for cellular transport of proteins and other biomacromolecules. During protein-mediated membrane fusion, membrane proteins are often excluded from the membrane-membrane contact, indicating that local structural transformations in lipid domains play a major role. However, the rearrangements of lipid domains during fusion have not been thoroughly examined. Here using a newly developed Fluorescence Surface Forces Apparatus (FL-SFA), migration of liquid-disordered clusters and depletion of liquid-ordered domains at the membrane-membrane contact are imaged in real time during hemifusion of model lipid membranes, together with simultaneous force-distance and lipid membrane thickness measurements. The load and contact time-dependent hemifusion results show that the domain rearrangements decrease the energy barrier to fusion, illustrating the significance of dynamic domain transformations in membrane fusion processes. Importantly, the FL-SFA can unambiguously correlate interaction forces and in situ imaging in many dynamic interfacial systems.
논문정보
- Research article
- 2015년 05월 (BRIC 등록일 2015-05-28)
- 국외 연구진
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