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한빛사논문
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Nat. Commun., Published 14 May 2015, 6, Article number: 7134 | doi:10.1038/ncomms8134
Green fluorescent protein nanopolygons as monodisperse supramolecular assemblies of functional proteins with defined valency
Abstract
Young Eun Kim1, Yu-na Kim1, Jung A Kim2, Ho Min Kim3 & Yongwon Jung1
1 Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea. 2 Graduate School of Nanoscience and Technology, KAIST, Daejeon 305-701 Korea. 3 Graduate School of Medical Science and Engineering, KAIST, Daejeon 305-701, Korea.
Correspondence to : Yongwon Jung
Abstract
Supramolecular protein assemblies offer novel nanoscale architectures with molecular precision and unparalleled functional diversity. A key challenge, however, is to create precise nano-assemblies of functional proteins with both defined structures and a controlled number of protein-building blocks. Here we report a series of supramolecular green fluorescent protein oligomers that are assembled in precise polygonal geometries and prepared in a monodisperse population. Green fluorescent protein is engineered to be self-assembled in cells into oligomeric assemblies that are natively separated in a single-protein resolution by surface charge manipulation, affording monodisperse protein (nano)polygons from dimer to decamer. Several functional proteins are multivalently displayed on the oligomers with controlled orientations. Spatial arrangements of protein oligomers and displayed functional proteins are directly visualized by a transmission electron microscope. By employing our functional protein assemblies, we provide experimental insight into multivalent protein-protein interactions and tools to manipulate receptor clustering on live cell surfaces.
논문정보
- Research article
- 2015년 05월 (BRIC 등록일 2015-05-20)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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