한빛사
>
한빛사논문
한빛사논문
- 조회4,052
J. Am. Chem. Soc., April 13, 2015 | DOI: 10.1021/ja5115776
Glucagon-Secreting Alpha Cell Selective Two-Photon Fluorescent Probe TP-α: For Live Pancreatic Islet Imaging
Abstract
Bikram Keshari Agrawalla†, Yogeswari Chandran‡, Wut-Hmone Phue†, Sung-Chan Lee‡, Yun-Mi Jeong‡, Si Yan Diana Wan‡, Nam-Young Kang*‡, and Young-Tae Chang*†‡
† Department of Chemistry & Medicinal Chemistry Program of Life Sciences Institute, National University of Singapore, Singapore 117543, Singapore
‡ Laboratory of Bio-imaging Probe Development, Singapore Bio-imaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore
*Correspondence to : Nam-Young Kang, Young-Tae Chang
Abstract
Two-photon (TP) microscopy has an advantage for live tissue imaging which allows a deeper tissue penetration up to 1 mm comparing to one-photon (OP) microscopy. While there are several OP fluorescence probes in use for pancreatic islet imaging, TP imaging of selective cells in live islet still remains a challenge. Herein, we report the discovery of first TP live pancreatic islet imaging probe; TP-α (Two Photon-alpha) which can selectively stain glucagon secreting alpha cells. Through fluorescent image based screening using three pancreatic cell lines, we discovered TP-α from a TP fluorescent dye library TPG (TP-Green). In vitro fluorescence test showed that TP-α have direct interaction and appear glucagon with a significant fluorescence increase, but not with insulin or other hormones/analytes. Finally, TP-α was successfully applied for 3D imaging of live islets by staining alpha cell directly. The newly developed TP-α can be a practical tool to evaluate and identify live alpha cells in terms of localization, distribution and availability in the intact islets.
논문정보
- Research article
- 2015년 04월 (BRIC 등록일 2015-04-24)
- 국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
