구.농수식품
- 조회3,481
Abstract
Jeong-Hwan Mun1, Hee Chung2, Won-Hyong Chung3, Mijin Oh4, Young-Min Jeong2, Namshin Kim3, Byung Ohg Ahn4, Beom-Seok Park5, Suhyoung Park6, Ki-Byung Lim7, Yoon-Jung Hwang8, Hee-Ju Yu2,*
1 Department of Bioscience and Bioinformatics, Myongji University, Yongin, 449-728, Korea
2 Department of Life Science, The Catholic University of Korea, Bucheon, 420-743, Korea
3 Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea
4 Department of Genomics, National Academy of Agricultural Science, Rural Development Administration, Wanju, 565-851, Korea
5 The Agricultural Genome Center, National Academy of Agricultural Science, Rural Development Administration, Wanju, 565-851, Korea
6 Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon, 440-706, Korea
7 Department of Horticultural Science, Kyungpook National University, Daegu, 702-701, Korea
8 Department of Life Science, Sahmyook University, Seoul, 139-800, Korea
* Corresponding author
Abstract
Raphanus sativus is an annual vegetable crop species of the Brassicaceae family and is one of the key plants in the seed industry, especially in East Asia. Assessment of the R. sativus genome provides fundamental resources for crop improvement as well as the study of crop genome structure and evolution. With the goal of anchoring genome sequence assemblies of R. sativus cv. WK10039 whose genome has been sequenced onto the chromosomes, we developed a reference genetic map based on genotyping of two parents (maternal WK10039 and paternal WK10024) and 93 individuals of the F2 mapping population by whole-genome resequencing. To develop high-confidence genetic markers, ~83 Gb of parental lines and ~591 Gb of mapping population data were generated as Illumina 100 bp paired-end reads. High stringent sequence analysis of the reads mapped to the 344 Mb of genome sequence scaffolds identified a total of 16,282 SNPs and 150 PCR-based markers. Using a subset of the markers, a high-density genetic map was constructed from the analysis of 2,637 markers spanning 1,538 cM with 1,000 unique framework loci. The genetic markers integrated 295 Mb of genome sequences to the cytogenetically defined chromosome arms. Comparative analysis of the chromosome-anchored sequences with Arabidopsis thaliana and Brassica rapa revealed that the R. sativus genome has evident triplicated sub-genome blocks and the structure of gene space is highly similar to that of B. rapa. The genetic map developed in this study will serve as fundamental genomic resources for the study of R. sativus.
논문정보
- Research article
- 2015년 02월 (BRIC 등록일 2015-02-02)
- 국내 연구진
- 생명과학 > 유전학
- 구・농수식품
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