구.농수식품
- 조회2,047
Ehime University, Tokyo Medical and Dental University, Chubu University
Abstract
Ji-Won Lee1,2,3, Midori Asai3, Sang-Kyung Jeon4, Tadahiro Iimura1,5, Takayuki Yonezawa3, Byung-Yoon Cha3, Je-Tae Woo3,* and Akira Yamaguchi2
1 Division of Bio-Imaging, Proteo-Science Center (PROS), Ehime University, Ehime, Japan
2 Section of Oral Pathology, Graduate School of Medicine and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan
3 Research Institute for Biological Functions, Chubu University, Aichi, Japan
4 Food Science and Technology, Keimyung University, Daegu, Korea
5 Translational Research Center, Ehime University Hospital, Ehime, Japan
*Correspondence: Professor Je-Tae Woo, Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi 487-8501, Japan
Scope
Bone homeostasis is ensured by the balance between bone formation and resorption. Thus, control of the recruitment, proliferation, and differentiation of bone cells is essential to maintain bone mass. The aim of this study was to elucidate the effects of rosmarinic acid as a potential therapeutic agent on bone metabolism using bone cells and a mouse model.
Methods and results
Rosmarinic acid increased alkaline phosphatase activity and induced mineralization in osteoblasts. Addition of rosmarinic acid to cultures of calvarial osteoblastic cells prepared from T-cell factor/β-catenin TOP-GAL mutant mice strongly induced the expression of LacZ and promoted stabilization of β-catenin in the cytoplasm of ST2 cells, suggesting that rosmarinic acid affects the canonical Wnt signaling pathway. Moreover, rosmarinic acid inhibited not only osteoclast formation in cocultures of mouse bone marrow cells and osteoblasts, but also receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastic differentiation in bone marrow-derived macrophages. RANKL-induced p38 mitogen-activated protein kinase and the expression of nuclear factor of activated T cell, c-Jun, and c-Fos were inhibited by rosmarinic acid in bone marrow macrophages. Finally, we confirmed that rosmarinic acid improved bone mass in a soluble RANKL-induced bone loss mouse model.
Conclusion
Rosmarinic acid has dual regulatory effects on bone metabolism and may control the bone functions by controlling osteoblastic and osteoclastic differentiation.
Keywords: β-Catenin; Osteoblast; Osteoclast; Rosmarinic acid; Wnt
논문정보
- Research article
- 2015년 01월 (BRIC 등록일 2015-01-26)
- 국내+국외 연구진
- 구・농수식품
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