구.추천논문
- 조회2,548
Abstract
Ji-Eun Kim, Somin Lee, Ah Young Lee, Hwi Won Seo, Chanhee Chae, and Myung-Haing Cho
1Laboratory of Toxicology, BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Korea,
2Graduate School of Convergence Science and Technology, Seoul National University, Suwon, Korea,
3Graduate Group of Tumor Biology, Seoul National University, Seoul, Korea,
4Laboratory of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Korea, and
5Advanced Institute of Convergence Technology, Seoul National University, Suwon, Korea
Correspondence: Myung-Haing Cho, Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Abstract
The effects of multi-walled carbon nanotubes (MWCNTs) exposure have garnered great interest in the field of public health, due to the high aspect ratio of MWCNTs. Because of worldwide increases in obesity prevalence, nonalcoholic fatty liver disease (NAFLD) is now the most common prevalent liver disease and is considered to be a component of metabolic syndrome, which is a cluster of disorders that also includes dyslipidemia, diabetes mellitus, arteriosclerosis, and hypertension. Exposure to MWCNTs is known to be a risk factor for lung and cardiovascular diseases, but its effect on NAFLD is unknown. In this study, we investigated the effects of intratracheal exposure of two different types of MWCNTs, namely, pristine multi-walled carbon nanotubes (PMWCNTs) and acid-treated multi-walled carbon nanotubes (TMWCNTs), on liver pathogenesis. Direct instillation of a test material into the lungs has been employed as a quantitatively reliable alternative method of inhalation exposure. The 10% weight loss dose was assessed in three months of subchronic study and is defined here as the maximum tolerated dose (MTD) of PMWCNTs and TMWCNTs; by this metric, MTD for a 1-year exposure of MWCNTs was determined to be 0.1 mg/mouse. Mice exposed to PMWCNTs and TMWCNTs for one year developed a nonalcoholic steatohepatitis (NASH)-like phenotype, characterized by inflammation, hepatic steatosis, and fibrosis. Furthermore, PMWCNTs induced a more severe NASH-like phenotype than TMWCNTs, which was related to consistent up-regulation of interleukin (IL)-6 and plasminogen activator inhibitor (PAI)-1. Impaired cholesterol homeostasis, overexpression of NF-κBp65, and suppression of peroxisome proliferator-activated receptor gamma (PPARγ) in the liver were also observed.
Keywords :Cholesterol, multi-walled carbon nanotubes, nonalcoholic fatty liver disease, risk factor
논문정보
- Research article
- 2014년 09월 (BRIC 등록일 2015-01-23)
- 국내 연구진
- 생명과학 > 발생생물학
- 구・추천논문
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