최학수 (Beth Israel Deaconess Medical Center, Harvard Medical School, 부산대학교) | 한빛사논문 > 한빛사

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Beth Israel Deaconess Medical Center, Harvard Medical School, 부산대학교

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Nat. Med., Published online 05 January 2015 | doi:10.1038/nm.3728 Structure-inherent targeting of near-infrared fluorophores for parathyroid and thyroid gland imaging

Abstract

Hoon Hyun^1,9, Min Ho Park^1,3,9, Eric A Owens^4,5, Hideyuki Wada¹, Maged Henary^4,5, Henricus J M Handgraaf⁶, Alexander L Vahrmeijer⁶, John V Frangioni^1,2,7 & Hak Soo Choi^1,8,*

¹Department of Medicine, Division of Hematology and Oncology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA. ²Department of Radiology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA. ³Department of Surgery, Chonnam National University Medical School, Gwangju, South Korea. ⁴Department of Chemistry, Georgia State University, Atlanta, Georgia, USA. ⁵Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, Georgia, USA. ⁶Department of Surgery, Leiden University Medical Center, Leiden, the Netherlands. ⁷Curadel, LLC, Worcester, Massachusetts, USA. ⁸Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, South Korea. ⁹These authors contributed equally to this work.

^*Correspondence to: Hak Soo Choi

Abstract
The typical method for creating targeted contrast agents requires covalent conjugation of separate targeting and fluorophore domains. In this study, we demonstrate that it is possible to create near-infrared (NIR) fluorophores with different tissue specificities driven by their inherent chemical structures. Thus, a single compact molecule performs both targeting and imaging. We use this strategy to solve a major problem in head and neck surgery: the identification and preservation of parathyroid and thyroid glands. We synthesized 700-nm and 800-nm halogenated fluorophores that show high uptake into these glands after a single intravenous (IV) injection of 0.06 mg kg-1 in a pig. By using a dual-channel NIR imaging system, we observed-in real time and with high sensitivity-the unambiguous distinction of parathyroid and thyroid glands simultaneously in the context of blood and surrounding soft tissue. This novel technology lays a foundation for performing head and neck surgery with increased precision and efficiency along with potentially lower morbidity, and it provides a general strategy for developing targeted NIR fluorophores.

논문정보

형식Research article
게재일2015년 01월 (BRIC 등록일 2015-01-08)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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