권성훈 (서울대학교, 기초과학연구원, 주식회사 퀀타매트릭스) | 한빛사논문 > 한빛사

한빛사논문, 상위피인용논문

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서울대학교, 기초과학연구원, 주식회사 퀀타매트릭스

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Sci. Transl. Med., 17 December 2014, Vol. 6, Issue 267, p. 267ra174 A rapid antimicrobial susceptibility test based on single-cell morphological analysis

Jungil Choi^1,2,3, Jungheon Yoo⁴, Mincheol Lee⁴, Eun-Geun Kim^1,2,3,4, Ji Soo Lee⁵, Seungok Lee⁶, Seik Joo⁷, Sang Hoon Song⁷, Eui-Chong Kim⁷, Jung Chan Lee^8,9,10, Hee Chan Kim^8,9,10, Yong-Gyun Jung^4,* and Sunghoon Kwon^1,2,3,4,*

¹Center for Nanoparticle Research, Institute for Basic Science, Seoul 151-742, Republic of Korea. ²Department of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-742, Republic of Korea. ³Bio-MAX Institute, Seoul National University, Seoul 151-742, Republic of Korea. ⁴QuantaMatrix Inc., Seoul 151-742, Republic of Korea. ⁵Interdisciplinary Program of Bioengineering, Seoul National University Graduate School, Seoul 151-742, Republic of Korea. ⁶Department of Laboratory Medicine, Incheon St. Mary’s Hospital, The Catholic University School of Medicine, Incheon 403-720, Republic of Korea. ⁷Department of Laboratory Medicine, Seoul National University Hospital, Seoul 110-744, Republic of Korea. ⁸Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul 110-799, Republic of Korea. ⁹Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea. ¹⁰Department of Biomedical Engineering, Seoul National University Hospital, Seoul 110-744, Republic of Korea.

^*Corresponding author.

Abstract

A rapid antibiotic susceptibility test (AST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs, which rely on cell culture, are not suitable for urgent cases of bacterial infection and antibiotic resistance owing to their relatively long test times. We describe a novel AST called single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by automatically analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. The SCMA was tested with four Clinical and Laboratory Standards Institute standard bacterial strains and 189 clinical samples, including extended-spectrum β-lactamase–positive Escherichia coli and Klebsiella pneumoniae, imipenem-resistant Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococci from hospitals. The results were compared with the gold standard broth microdilution test. The SCMA results were obtained in less than 4 hours, with 91.5% categorical agreement and 6.51% minor, 2.56% major, and 1.49% very major discrepancies. Thus, SCMA provides rapid and accurate antimicrobial susceptibility data that satisfy the recommended performance of the U.S. Food and Drug Administration.

논문정보

형식Research article
게재일2014년 12월 (BRIC 등록일 2014-12-22)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질
피인용횟수120회 이상 인용된 논문 (상위피인용논문 등록일 2020-11-12)

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