한빛사논문
- 조회1,326
Abstract
Linqing Feng1,3, Osung Kwon1.3, Bokyoung Lee1,2, Won Chan Oh1 & Jinhyun Kim1,2, *
1Center for Functional Connectomics, Korea Institute of Science and Technology (KIST), Seoul, Korea. 2Neuroscience program, University of Science and Technology, Daejeon, Korea. 3These authors contributed equally to this work.
*Correspondence to: Jinhyun Kim
Abstract
Many types of questions in neuroscience require the detection and mapping of synapses in the complex mammalian brain. A tool, mammalian GFP reconstitution across synaptic partners (mGRASP), offers a relatively easy, quick and economical approach to this technically challenging task. Here we describe in step-by-step detail the protocols for virus production, gene delivery, brain specimen preparation, fluorescence imaging and image analysis, calibrated substantially and specifically to make mGRASP-assisted circuit mapping (mGRASPing) practical in the mouse brain. The protocol includes troubleshooting suggestions and solutions to common problems. The mGRASP method is suitable for mapping mammalian synaptic connectivity at multiple scales: microscale for synapse-by-synapse or neuron-by-neuron analysis, and mesoscale for revealing local and long-range circuits. The entire protocol takes 5?6 weeks, including time for incubation and virus expression.
논문정보
- Research article
- 2014년 09월 (BRIC 등록일 2014-09-24)
- 국내 연구진
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