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ACS Nano, September 4, 2014 | DOI: 10.1021/nn5009269 Spraying Quantum Dot Conjugates in the Colon of Live Animals Enabled Rapid and Multiplex Cancer Diagnosis Using Endoscopy

Abstract

Youngrong Park^†^¶, Yeon-Mi Ryu^‡^¶, Yebin Jung^†, Taejun Wang^§, Yeonggyeong Baek^⊥, Yeoreum Yoon^∥, Sang Mun Bae^‡, Joonhyuck Park^†, Sekyu Hwang^†, Jaeil Kim^#, Eun-Ju Do^‡, Sang-Yeob Kim^‡∇, Euiheon Chung^◎, Ki Hean Kim^*§∥, Sungjee Kim^*†⊥, and Seung-Jae Myung^*‡○

^† Department of Chemistry, Pohang University of Science and Technology (POSTECH), San 31, Hyojadong, Nam-gu, Pohang 790-784, South Korea
^‡ Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro, 43-gil, Songpa-gu, Seoul 138-736, South Korea
^§ Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), San 31, Hyojadong, Nam-gu, Pohang 790-784, South Korea
^⊥ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), San 31, Hyojadong, Nam-gu, Pohang 790?784, South Korea
^∥ Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyojadong, Nam-gu, Pohang 790-784, South Korea
^# Health Screening and Promotion Center, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro, 43-gil, Songpa-gu, Seoul 138-736, South Korea
^∇ Department of Medicine, University of Ulsan College of Medicine, 88 Olympic-ro, 43-gil, Songpa-gu, Seoul 138-736, South Korea
^◎Department of Medical System Engineering and School of Mechatronics, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, South Korea
^○ Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro, 43-gil, Songpa-gu, Seoul 138-736, South Korea
^¶These authors contributed equally

^*Address correspondence to Ki Hean Kim, Sungjee Kim, Seung-Jae Myung

Abstract
The detection of colon cancer using endoscopy is widely used, but the interpretation of the diagnosis is based on the clinician’s naked eye. This is subjective and can lead to false detection. Here we developed a rapid and accurate molecular fluorescence imaging technique using antibody-coated quantum dots (Ab?QDs) sprayed and washed simultaneously on colon tumor tissues inside live animals, subsequently excited and imaged by endoscopy. QDs were conjugated to matrix metalloproteinases (MMP) 9, MMP 14, or carcinoembryonic antigen (CEA) Abs with zwitterionic surface coating to reduce nonspecific bindings. The Ab-QD probes can diagnose tumors on sectioned mouse tissues, fresh mouse colons stained ex vivo and also in vivo as well as fresh human colon adenoma tissues in 30 min and can be imaged with a depth of 100 μm. The probes successfully detected not only cancers that are readily discernible by bare eyes but also hyperplasia and adenoma regions. Sum and cross signal operations provided postprocessed images that can show complementary information or regions of high priority. This multiplexed quantum dot, spray-and-wash, and endoscopy approach provides a significant advantage for detecting small or flat tumors that may be missed by conventional endoscopic examinations and bestows a strategy for the improvement of cancer diagnosis.

Keywords: quantum dot; imaging probe; nanoparticle; colon cancer; tumor imaging; endoscopy

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형식Research article
게재일2014년 09월 (BRIC 등록일 2014-09-19)
연구진국내 연구진
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