상위피인용논문
- 조회948
Abstract
Woo Kyung Jung1,†, Hye Cheong Koo1,2,*, Ki Woo Kim3, Sook Shin1, So Hyun Kim1 and Yong Ho Park1,*
1Department of Microbiology
2KRF Zoonotic Disease Priority Research Institute, College of Veterinary Medicine and BK21 Program for Veterinary Science
3National Instrumentation Center for Environmental Management, Seoul National University, Seoul, Korea
*Corresponding authors.
†Present address: Samsung Electronics Co. Ltd., Suwon, Korea.
ABSTRACT
The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log10 CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.
논문정보
- Research article
- 2008년 04월 (BRIC 등록일 2014-02-28)
- 국내 연구진
- 120회 이상 인용된 논문
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