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Abstract
Sung Ho Yang†, Sung Min Kang †‡, Kyung-Bok Lee §, Taek Dong Chung∥, Haeshin Lee †‡, and Insung S. Choi *†¶
† Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon 305-701, Korea
‡ The Graduate School of Nanoscience and Technology, KAIST, Daejeon 305-701, Korea
§ Division of Life Science, Korea Basic Science Institute (KBSI), Daejeon 305-333, Korea
∥ Department of Chemistry, Seoul National University, Seoul 151-747, Korea
¶ Department of Bio and Brain Engineering, KAIST, Daejeon 305-701, Korea
*Correspondence to: Insung S. Choi
Abstract
The individual encapsulation of living cells has a great impact on the area of cell-based sensors and devices as well as fundamental studies in cell biology. In this work, living yeast cells were individually encapsulated with functionalizable, artificial polydopamine shells, inspired by an adhesive protein in mussels. Yeast cells maintained their viability within polydopamine, and the cell cycle was controlled by the thickness of the shells. In addition, the artificial shells aided the cell in offering much stronger resistance against foreign aggression, such as lyticase. After formation of the polydopamine shells, the shells were functionalized with streptavidin by utilizing the chemical reactivity of polydopamine, and the functionalized cells were biospecifically immobilized onto the defined surfaces. Our work suggests a biomimetic approach to the encapsulation and functionalization of individual living cells with covalently bonded, artificial shells.
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