상위피인용논문
- 조회2,144
Abstract
Mi Hyang Moon1*, Sun Young Kim2*, Yeon Jeong Kim1, Su Jin Kim1, Jae Bong Lee3, Yong Chan Bae4, Sang Min Sung5 and Jin Sup Jung1,6
1Department of Physiology, College of Medicine, Pusan National University, 2Department of Pediatrics, College of Medicine, Soonchunhyang University, 3Beautis Derm Surg Center, 4Department of Plastic Surgery, College of Medicine, Pusan National University, 5Department of Neurology, Pusan medical center, 6Medical Research Center for Ischemic Tissue Regeneration, Pusan National University, *These authors equally contributed in the work
Corresponding author : Jin Sup Jung, MD, PhD.
Abstract
Background/Aim: It has been reported that adipose tissue contain progenitor cells with angiogenic potential and that therapy based on adipose tissuederived progenitor cells administration may constitute a promising cell therapy in patients with ischemic disease. In this study we evaluated the effect of culture-expanded mesenchymal stem cells (MSC) derived from adipose tissue on neovascularization and blood flow in an animal model of limb ischemia in immunodeficient mice. Methods: MSC were cultured from human adipose tissue by collagenase digestion. Hindlimb ischemia was created by ligating the proximal femoral artery of male nude mice. Human adipose tissue stromal cells (hADSC) were transplanted one day or 7 days after ligation. Results: During culture expansion of hADSC CD34 expression was downregulated. The laser Doppler perfusion index was significantly higher in the CD34(-), Flk-1(-), CD31(-) ADSC-transplanted group than in the control group, even when cells were transplanted 7days after hindlimb ischemia. Histological examination showed that hADSC transplantation recovered muscle injury and increased vascular density, compared with the control group. The effect of hADSC was correlated with the number of transplanted cells, but not with the ratio of CD34 expression. In vitro, hADSC can form vessel-like structure and express von Willibrand Factor. Conditioned media from hADSC increased proliferation and inhibited apoptotic cell death in of human aortic endothelial cells. Conclusion: This study showed that hADSC can be an ideal source for therapeutic angiogenesis in ischemic disease.
Key Words : Vasculogenesis, Endothelium, Human adipose stromal cells, Hindlimb ischemia
논문정보
- Research article
- 2006년 03월 (BRIC 등록일 2013-08-19)
- 국내 연구진
- 의약학 > 인체시스템의학
- 120회 이상 인용된 논문
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