이준철 (성균관대학교 의과대학) | 한빛사논문 > 한빛사

한빛사논문, 상위피인용논문

조회4,728

성균관대학교 의과대학

CV File 120 kb

Gut, Published Online First 26 June 2013. Doi:10.1136/gutjnl-2012-303839 An increase in the Akkermansia spp. population induced by metformin treatment improves glucose homeostasis in diet-induced obese mice

Abstract

Na-Ri Shin¹, June-Chul Lee², Hae-Youn Lee², Min-Soo Kim¹, Tae Woong Whon¹, Myung-Shik Lee², Jin-Woo Bae¹

¹Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul, Korea
²Department of Medicine and Department of Biotechnology & Bioengineering, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
Correspondence to Dr Jin-Woo Bae or Dr Myung-Shik Lee

Abstract
Background Recent evidence indicates that the composition of the gut microbiota contributes to the development of metabolic disorders by affecting the physiology and metabolism of the host. Metformin is one of the most widely prescribed type 2 diabetes (T2D) therapeutic agents.

Objective To determine whether the antidiabetic effect of metformin is related to alterations of intestinal microbial composition.

Design C57BL/6 mice, fed either a normal-chow diet or a high-fat diet (HFD), were treated with metformin for 6 weeks. The effect of metformin on the composition of the gut microbiota was assessed by analysing 16S rRNA gene sequences with 454 pyrosequencing. Adipose tissue inflammation was examined by flow cytometric analysis of the immune cells present in visceral adipose tissue (VAT).

Results Metformin treatment significantly improved the glycaemic profile of HFD-fed mice. HFD-fed mice treated with metformin showed a higher abundance of the mucin-degrading bacterium Akkermansia than HFD-fed control mice. In addition, the number of mucin-producing goblet cells was significantly increased by metformin treatment (p<0.0001). Oral administration of Akkermansia muciniphila to HFD-fed mice without metformin significantly enhanced glucose tolerance and attenuated adipose tissue inflammation by inducing Foxp3 regulatory T cells (Tregs) in the VAT.

Conclusions Modulation of the gut microbiota (by an increase in the Akkermansia spp. population) may contribute to the antidiabetic effects of metformin, thereby providing a new mechanism for the therapeutic effect of metformin in patients with T2D. This suggests that pharmacological manipulation of the gut microbiota in favour of Akkermansia may be a potential treatment for T2D.

논문정보

TOP52013년 선정

형식Research article
게재일2013년 06월 (BRIC 등록일 2013-06-28)
연구진국내 연구진
분야 생명과학 > 미생물학
피인용횟수60회 이상 인용된 논문 (상위피인용논문 등록일 2015-09-25)

댓글 작성 로그인

CV 열람하기

연락처 보기