조종수 (서울대학교) | 한빛사논문 > 한빛사

상위피인용논문

조회1,369

서울대학교

CV File 385 kb

Biomaterials, Volume 25, Issue 17, August 2004, Pages 3783–3792 | http://dx.doi.org/10.1016/j.biomaterials.2003.10.063 Galactosylated chitosan/DNA nanoparticles prepared using water-soluble chitosan as a gene carrier

Abstract

Tae Hee Kim^a, In Kyu Park^a, Jae Woon Nah^b, Yun Jaie Choi^a, Chong Su Cho^a

^a School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea
^b Department of Polymer Science and Engineering, Sunchon National University, Sunchon 540-742, South Korea

^*Corresponding author : Chong Su Cho

Abstract
Water-soluble chitosan (WSC) was used to increase the stability of chitosan in water and decrease the cytotoxicity induced by acetic acid. Lactobionic acid (LA) bearing galactose group was coupled with WSC for hepatocytes specificity. The composition of galactose in galactosylated chitosan (GC) was determined by NMR spectroscopy. The GC was complexed with plasmid DNA in various GC/DNA (N/P) charge ratios and the resulting complex was characterized by dynamic light scattering, gel retardation, and turbidity to determine the particle sizes, complex formation, and complex stability, respectively. Cytotoxicity and transfection efficiency of GC were also studied in cultured HepG2 human hepatoblastoma cell line and HeLa human cervix epithelial carcinoma cells. The complete GC/DNA complex was formed at the charge ratio of 5 and the GC/DNA complex to DNase I resistance was obtained. Particle sizes decreased with increasing charge ratio of GC to DNA and had a minimum value around 120 nm at the charge ratio of 5. And no significant difference in particle sizes from the charge ratio of 5-20 was found. The suspension of GC/DNA complexes exhibited no significant change in turbidity at the charge ratios of 10, indicating the complete shielding of DNA charge. Cytotoxicity study showed that GC prepared by the water-soluble chitosan had no cytotoxic effects on cells. And transfection efficiency into HepG2, which has asialoglycoprotein receptors (ASGP-R), was higher than that into HeLa without ASGP-R.

Keywords : Gene carrier; Cationic polymer; Water-soluble chitosan; Galactosylated chitosan/DNA nanoparticles; Asialoglycoprotein receptor

논문정보

형식Research article
게재일2004년 08월 (BRIC 등록일 )
연구진국내 연구진
분야
피인용횟수120회 이상 인용된 논문

댓글 작성 로그인

CV 열람하기

연락처 보기