Hongje Jang3, Soo-Ryoon Ryoo1, Young-Kwan Kim1, Soojin Yoon2, Henna Kim3, Prof. Dr. Sang Woo Han3, Prof. Dr. Byong-Seok Choi3, Prof. Dr. Dong-Eun Kim2, Prof. Dr. Dal-Hee Min1,*
1 Department of Chemistry, Seoul National University, Seoul, 151-747 (Korea)
2 Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701 (Korea)
3 Department of Chemistry, KAIST, Daejeon, 305-701 (Korea)
*To whom correspondence may be addressed.
† This work was supported by the Basic Science Research Program (2011-0017356 for D.-H. M., 2009-0092818, 2011-0020322 for B.-S. C. and 2010-0019306, 2011-0016385 for D.-E. K.), the EPB Center (2008-0062042), and by the Research Center Program (EM1202) of the Institute for Basic Science (D.-H. M.) through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST).
A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.
Keywords: biosensors; graphene oxide; high-throughput screening; helicases; multiplex assays