한빛사논문
- 조회2,290
Abstract
Min-Young Lee†, Jeong-A Yang †, Ho Sang Jung†, Songeun Beack†, Jung Eun Choi‡, Wonhee Hur ‡, Heebeom Koo§, Kwangmeyung Kim§, Seung Kew Yoon‡, and Sei Kwang Hahn†*
† Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea
‡ Department of Internal Medicine and WHO Collaborating Center of Viral Hepatitis, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea
§ Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Korea
*correspondence to : Sei Kwang Hahn
Abstract
Gold nanoparticles (AuNPs) have been extensively investigated as an emerging delivery carrier of various biopharmaceuticals. Instead of nonspecific polyethylene glycol (PEG) conjugated interferon α (IFNα) for the clinical treatment of hepatitis C virus (HCV) infection, in this work, a target-specific long-acting delivery system of IFNα was successfully developed using the hybrid materials of AuNP and hyaluronic acid (HA). The HA?AuNP/IFNα complex was prepared by chemical binding of thiolated HA and physical binding of IFNα to AuNP. According to antiproliferation tests in Daudi cells, the HA?AuNP/IFNα complex showed a comparable biological activity to PEG-Intron with a highly enhanced stability in human serum. Even 7 days postinjection, HA?AuNP/IFNα complex was target-specifically delivered and remained in the murine liver tissue, whereas IFNα and PEG-Intron were not detected in the liver. Accordingly, HA?AuNP/IFNα complex significantly enhanced the expression of 2′,5′-oligoadenylate synthetase 1 (OAS1) for innate immune responses to viral infection in the liver tissue, which was much higher than those by IFNα, PEG-Intron, and AuNP/IFNα complex. Taken together, the target-specific HA?AuNP/IFNα complex was thought to be successfully applied to the systemic treatment of HCV infection.
Keywords: gold nanoparticle; hyaluronic acid; interferon α; targeted delivery; hepatitis C virus
논문정보
- Research article
- 2012년 10월 (BRIC 등록일 )
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
