이상우 (연세대학교) | 한빛사논문 > 한빛사

한빛사논문

조회1,190

연세대학교

ACS Nano, 2012, 6 (10), pp 8665–8673. Publication Date (Web): September 11, 2012 | DOI: 10.1021/nn302202t Microfluidic Multifunctional Probe Array Dielectrophoretic Force Spectroscopy with Wide Loading Rates

Abstract

In Soo Park^†, Kilho Eom^†, Jongsang Son^†, Woo-Jin Chang^‡, Kidong Park^§, Taeyun Kwon^†, Dae Sung Yoon^†, Rashid Bashir^*§, and Sang Woo Lee^*†

^† Department of Biomedical Engineering, Yonsei University, Won-Ju, 220-710, Korea
^‡ Department of Mechanical Engineering, Universiyt of Wisconsin?Milwaukee, Milwaukee, Wisconsin 53201, United States
^§ Department of Electrical and Computer Engineering, Department of Bioengineering, and Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Illinois 61801, United States

*correspondence to Sang Woo Lee, Rashid Bashir

Abstract
The simultaneous investigation of a large number of events with different types of intermolecular interactions, from nonequilibrium high-force pulling assays to quasi-equilibrium unbinding events in the same environment, can be very important for fully understanding intermolecular bond-rupture mechanisms. Here, we describe a novel dielectrophoretic force spectroscopy technique that utilizes microsized beads as multifunctional probes for parallel measurement of intermolecular forces with an extremely wide range of force rate (10^-4 to 10⁴ pN/s) inside a microfluidic device. In our experiments, various forces, which broadly form the basis of all molecular interactions, were measured across a range of force loading rates by multifunctional probes of various diameters with a throughput of over 600 events per mm2, simultaneously and in the same environment. Furthermore, the individual bond-rupture forces, the parameters for the characterization of entire energy landscapes, and the effective stiffness of the force spectroscopy were determined on the basis of the measured results. This method of determining intermolecular forces could be very useful for the precise and simultaneous examination of various molecular interactions, as it can be easily and cost-effectively implemented within a microfluidic device for a range of applications including immunoassays, molecular mechanics, chemical and biological screening, and mechanobiology.

Keywords: dielectrophoresis; force spectroscopy; intermolecular interactions; microfluidic device; quasi-equilibrium unbinding events; high-force pulling assay

논문정보

형식Research article
게재일2012년 09월 (BRIC 등록일 )
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

댓글 작성 로그인

CV 열람하기

연락처 보기