한빛사논문, 상위피인용논문
- 조회2,391
Abstract
Seung-Hee Lee1,2, Alex C. Kwan1, Siyu Zhang1, Victoria Phoumthipphavong1, John G. Flannery1, Sotiris C. Masmanidis3, Hiroki Taniguchi4, Z. Josh Huang4, Feng Zhang5, Edward S. Boyden6, Karl Deisseroth5 & Yang Dan1,2,*
1Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, University of California, Berkeley, California 94720, USA. 2Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA. 3Broad Fellows Program in Brain Circuitry, Kavli Nanoscience Institute, California Institute of Technology, Pasadena, California 91125, USA. 4Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA. 5Department of Bioengineering, Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA. 6Media Laboratory, Biological Engineering, Brain and Cognitive Sciences, and McGovern Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
*Correspondence to: Yang Dan
Inhibitory interneurons are essential components of the neural circuits underlying various brain functions. In the neocortex, a large diversity of GABA (γ-aminobutyric acid) interneurons has been identified on the basis of their morphology, molecular markers, biophysical properties and innervation pattern1, 2, 3. However, how the activity of each subtype of interneurons contributes to sensory processing remains unclear. Here we show that optogenetic activation of parvalbumin-positive (PV+) interneurons in the mouse primary visual cortex (V1) sharpens neuronal feature selectivity and improves perceptual discrimination. Using multichannel recording with silicon probes4, 5 and channelrhodopsin-2 (ChR2)-mediated optical activation6, we found that increased spiking of PV+ interneurons markedly sharpened orientation tuning and enhanced direction selectivity of nearby neurons. These effects were caused by the activation of inhibitory neurons rather than a decreased spiking of excitatory neurons, as archaerhodopsin-3 (Arch)-mediated optical silencing7 of calcium/calmodulin-dependent protein kinase IIα (CAMKIIα)-positive excitatory neurons caused no significant change in V1 stimulus selectivity. Moreover, the improved selectivity specifically required PV+ neuron activation, as activating somatostatin or vasointestinal peptide interneurons had no significant effect. Notably, PV+ neuron activation in awake mice caused a significant improvement in their orientation discrimination, mirroring the sharpened V1 orientation tuning. Together, these results provide the first demonstration that visual coding and perception can be improved by increased spiking of a specific subtype of cortical inhibitory interneurons.
논문정보
- Research article
- 2012년 08월 (BRIC 등록일 )
- 국외 연구진
- 생명과학 > 신경생물학
- 120회 이상 인용된 논문 (상위피인용논문 등록일 2018-10-08)
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