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Adv. Funct. Mater., Volume 22, Issue 21, pages 4448–4455, November 7, 2012. DOI: 10.1002/adfm.201200657 Ethanol-Dispersed Polymer Nanofi bers as a Highly Selective Cell Isolation and Release Platform for CD4 + T Lymphocytes

Abstract

Seung-Hyun Jun^1,†, Kwanghee Kim^2,†, Hyo Jin An¹, Byoung Chan Kim³, Chung Hee Sonn², Miju Kim^4,5, Junsang Doh^4,5, Cassian Yee⁶, Kyung-Mi Lee^2,*, Jungbae Kim^1,*

¹Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
²Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Republic of Korea
³Environment Sensor System Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea
⁴Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
⁵School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
⁶Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 USA

^*Corresponding authors :Kyung-Mi Lee, Jungbae Kim
^†Seung-Hyun Jun and Kwanghee Kim contributed equally to this work.

Abstract
A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4⁺ T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4⁺ T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4⁺ T cells for the purpose of cell therapies.

Keywords:electrospun polymer nanofibers;ethanol dispersion;CD4+ T lymphocyte;cell separation;cell transplantation

논문정보

형식Research article
게재일2012년 07월 (BRIC 등록일 2012-07-23)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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