Seung-Hyun Jun1,†, Kwanghee Kim2,†, Hyo Jin An1, Byoung Chan Kim3, Chung Hee Sonn2, Miju Kim4,5, Junsang Doh4,5, Cassian Yee6, Kyung-Mi Lee2,*, Jungbae Kim1,*
1Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
2Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 136-713, Republic of Korea
3Environment Sensor System Research Center, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea
4Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
5School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea
6Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 USA
*Corresponding authors :Kyung-Mi Lee, Jungbae Kim
†Seung-Hyun Jun and Kwanghee Kim contributed equally to this work.
A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4+ T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4+ T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4+ T cells for the purpose of cell therapies.
Keywords:electrospun polymer nanofibers;ethanol dispersion;CD4+ T lymphocyte;cell separation;cell transplantation