한빛사논문
- 조회7,385
Abstract
Kyoung G. Lee†¶, Jongin Hong‡, Kye Won Wang†, Nam Su Heo§, Do Hyun Kim†, Sang Yup Lee†§, Seok Jae Lee¶*, and Tae Jung Park§¶*
†Department of Chemical & Biomolecular Engineering (BK21) and §BioProcess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea
‡ Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea
¶ Center for Nanobio Integration & Convergence Engineering, National Nanofab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea
*Address correspondence to Seok Jae Lee, Tae Jung Park
Abstract
We report the use of a hydrogel polymer, recombinant Escherichia coli cell extracts, and a microdroplet-based microfluidic device to fabricate artificial cellular bioreactors which act as reactors to synthesize diverse metal nanoparticles (NPs). The combination of cell extracts, microdroplet-based microfluidic device, and hydrogel was able to produce a mass amount of artificial cellular bioreactors with uniform size and shape. For the first time, we report the alternating generation of microdroplets through one orifice for the fabrication of the artificial cellular reactors using the cell extract as inner cellular components and hydrogel as an artificial cellular membrane. Notably, the hydrogels were able to protect the encapsulated cell extracts from the surrounding environment and maintain the functionality of cellular component for the further cellular bioreactor applications. Furthermore, the successful applications of the fabricated artificial cellular bioreactors to synthesize various NPs including quantum dots, iron, and gold was demonstrated. By employing this microfluidic technique, the artificial cellular bioreactors could be applicable for the synthesis of diverse metal NPs through simple dipping of the reactors to the metal precursor solutions. Thus, the different size of NPs can be synthesized through controlling the concentration of metal precursors. This artificial cellular bioreactors offer promising abilities to biofriendly ways to synthesis diverse NPs and can be applicable in chemical, biomedical, and bioengineering applications.
Keywords: microfluidics; artificial cellular reactor; in vitro biosynthesis; metal-binding proteins; nanoparticles
논문정보
- Research article
- 2012년 07월 (BRIC 등록일 )
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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